In the following, the online help texts for all data entry fields provided with any Endpoint summary record for this IUCLID section are listed. As to whether and to what extent endpoint summaries should be prepared, refer to the relevant guidance for the respective chemical programme, e.g., the Technical Guidance Document (TGD) on preparing the Chemical Safety Report under REACH in its most recent version.
For technical guidance on how to manage Endpoint summary records, see How to manage Endpoint summary records in sections 4 - 10, respectively. For details on data types, see What data types are available for input fields and how are they used?.
In the following, the online help texts for all data entry fields provided with any Endpoint summary record for this IUCLID section are listed. As to whether and to what extent endpoint summaries should be prepared, refer to the relevant guidance for the respective chemical programme, e.g., the Technical Guidance Document (TGD) on preparing the Chemical Safety Report under REACH in its most recent version.
For technical guidance on how to manage Endpoint summary records, see How to manage Endpoint summary records in sections 4 - 10, respectively. For details on data types, see What data types are available for input fields and how are they used?.
Enter a full short description of the most relevant endpoint data. This includes in principle the summary information that is compiled e.g. in the OECD Full SIDS Summary format or other endpoint summary formats. Provide only the most relevant details, which could be, depending on the cases, the test guideline used, test organism and exposure duration. Normally no reliability score needs to be indicated as it can be assumed that the studies identified are valid (reliability score 1 or 2). If this is not the case (for instance if the reliability of a published study cannot be assigned or if several less valid studies are used for a weight of evidence analysis), the reliability indicators should be specified.
Also several key studies can be referenced as applicable. However, the characterisation of the endpoint data should be kept as concise as possible. Examples of what could be entered here are as follows:
- Melting point: 54.6-55.8 °C at 1,013 hPa
- Water solubility: completely miscible
- pH: 6.9 (80 mg/l water) at 20°C (OECD TG105)
- Oxidation reduction potential: no data available
- Phototransformation in air: T1/2 = 9.32 x 10-2 yr (sensitizer: OH radical) (AOP Win v 1.86)
- Biodegradation in water: screening tests: Not readily biodegradable: 0 - 8% (BOD) in 28 days, 0 - 1% (HPLC) in 28 days (OECD TG 301C)
- Short-term toxicity to fish:
LC50 (96h) < 100 mg/l for Pimephales promelas (OECD TG 203)
LC50 (48h) = 3.2 mg/l for Oryzias latipes (OECD TG 203)
- Acute toxicity:
Dermal: LD50: > 2,000 mg/kg for rat (limit test)
- Genetic toxicity:
In vitro:
Gene mutation (Bacterial reverse mutation assay / Ames test): S. typhimurium TA 100: positive with and without metabolic activation; TA 1535: positive without metabolic activation (equivalent to OECD TG 471)
The field(s) under this heading (if provided) can be optionally completed in order to specify the key parameter(s) that may subsequently be used in the chemical safety assessment, classification and labelling or other. In order to enable the use of any specific software, only a minimum number of structured and hence, searchable fields are provided, in many cases only one.
Key parameters are intended to condense the data summarised in field "Full short description of relevant endpoint data" to one single numeric value or concluding remark (e.g. negative / positive) chosen from a drop-down list. Where a numeric field is provided, only a clear value can be entered, that is, no range and no less than or greater than qualifiers. Conversion to a predefined unit as indicated in the field label (e.g. mg/L) may be required.
If the key value is no clear number, but a range or preceded by <, <=, >, or >=, you may either leave this field empty or make up a value you consider most appropriate for the intended subsequent purpose. The rationale for any user-derived values should be described in field "Discussion" for the sake of transparency. Some non-exhaustive examples of user-derived parameters are as follows:
- Aquatic short-term L(E)C50 >100 mg/L (e.g. because only tested up to water solubility of the substance): it may be appropriate to assume 100 mg/L as worst case value.
- Aquatic long-term LOEC 1 mg/L (>10 and <20% effect): calculate NOEC as LOEC/2 and enter 0.5 mg/L in the field for NOEC.
- Acute oral LD50 >2000 mg/kg b.w. (because no LD50 was achieved in a limit test): enter 2000 mg/kg b.w.
In this rich text field, describe the assessment you have made for the given endpoint. Provide the rationale for the choice of the key study(ies) and the choice of the key parameter that, according to your judgement, characterise the endpoint. This includes a discussion of the key information identified and in some instances of studies which are considered to be unreliable, but give critical results. A discussion as to why they were discarded in favour of other studies should then be included. Vice versa, a weight of evidence analysis based on less reliable data or use of published data, the reliability of which cannot be judged because of limited reporting, should be justified.
If several studies were identified to be relevant for the assessment, discuss possible reasons for differing results if any, e.g. differences in purity / impurities of the test substance used, differences in the methods and test conditions, etc.
In the following, the online help texts for all data entry fields provided with any Endpoint study record for this IUCLID section are listed. For sections 4 to 10, these guidance notes are completely based on the so-called OECD Harmonised Templates (see Rationale behind IUCLID Endpoint Study Records - OECD harmonised templates in chapter chapter D.4.7.1 What is an Endpoint study record?)
IUCLID per se does not prescribe how detailed the study summaries should be recorded. Refer to the relevant guidance for the respective chemical regulatory programme thereof.
For technical guidance on how to manage Endpoint study records, see chapter D.4.7 How to manage Endpoint study records in sections 4 - 13. For details on data types, see chapter D.4.5 What data types are available for input fields and how are they used?
Under this main heading, fields are subsumed for identifying the purpose of the record (e.g., "key study"), the type of result (e.g., "experimental study"), data waiving indication (if any), reliability indication, and flags for indicating the regulatory purpose envisaged and/or any confidentiality restrictions. This kind of data characterise the relevance of a study summary and are therefore displayed on top of each Endpoint Study Record. For detailed guidance, refer to chapter D.4.7.7.1 Administrative data.
Indicate the bibliographic reference of the study report or publication the study summary is based on. Always enter the primary reference in the first block of fields (i.e. Sort no. = 1), if there are more than one reference to be cited. Copy this block of fields for specifying any other references related to this record (e.g. report of a preliminary study or other documentation). If results of a study report have been published, indicate the full citation of that publication(s) in addition to the reference of the original study.
Table E.169. Field Descriptions
| Reference type |
Indicate the type of reference, e.g. "Study report" or "Publication". Select "Other company data" to characterise any unpublished information from a company other than a study report. Select "Grey literature" for any other unpublished information or "other:" and specify. |
| Author(s) (or transferred reference) (Author) |
For ease of sorting and searchability use following convention: Surname, Initial (Example 1: White D, Ruehl KJ, Borman SA & Little J. Example 2: Hartley M & Murray W (avoid unnecessary full-stops, commas)). If no individuals are cited as authors, enter name of company or organisation or "Anon." as appropriate. Note that the complete bibliographic reference may appear in this field after migration of unstructured data from existing databases. |
| Year |
Enter year of study report or publication. For a study report this field should be completed to include it in any searches, regardless of whether the complete date is given in field "Report date". |
| Title |
Include the title of the report. For publications, include the title of the article of a journal or article/chapter of a book (e.g. handbook). |
| Bibliographic source |
Not relevant for any study report. For publications or any other literature source (grey literature) specify the following type of information: (i) Title of scientific journal or book (e.g. if handbook); (ii) Volume of journal; (iii) Editor, publisher, place of publication for books or articles in books; (iv) Pagination. Example 1 (journal): J. Agric. Food Chem. 38: 215-227 Example 2 (handbook): In: Lyman WJ (ed.) Handbook of chemical property estimation methods. Environmental behavior of organic compounds. McGraw-Hill Book Company 15.1-15.34, New York. |
| Testing laboratory |
Either manually enter the name of the testing laboratory or select it from the picklist. In either case, editing is possible. |
| Report no. |
Specify the report number allocated by the testing laboratory. Note that any company-specific study number should be included in the respective field. |
| Owner company |
Either manually enter the identity of the company who owns the data or select it from the picklist. In either case, editing is possible. |
| Company study no. |
Specify any company study no. if there is such a number and if it is different from the report no. of the testing laboratory. Otherwise leave field empty. |
| Report date |
Specify the complete date of the study report, e.g. "2005-05-12" for 12 May 2005. Note that subfield "Year" should be completed in any case for sorting and searching purposes. |
Select appropriate indication for data access. Enter "Not applicable" if the summary consists of information that is commonly accessible such as guidance on safe use.
Indicate as appropriate. Note: "yes" should be selected only if "Data submitter is data owner" or "Data submitter has Letter of Access". Options "yes, but willing to share" or "yes, but not willing to share" may be relevant for specific regulatory programmes where the submitter is requested to indicate whether he is willing to share studies (e.g. with vertebrates).
In the supplementary remarks field, include an explanation as appropriate, i.e. justification for denial of sharing the corresponding study or refer to a document attached that provides justification (e.g. "for justification see attached document X")
A cross-reference can be included to indicate that the same study is recorded in another record. Indicate the respective chapter and record ID and enter relevant explanatory text. This may be useful if specific endpoints of a given study are described in another chapter (e.g. results on reproduction toxicity in case of a combined repeated dose / reproduction toxicity study) or if more than one experiment is described by the same study report, but included in separate records.
Check with the relevant guidance document whether all the methodology details must be repeated or whether a cross-reference to the same study in another chapter may suffice.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
Indicate whether the study was a ready biodegradability test or inherent biodegradability test. If "other:" is selected, specify.
NOTE: Any simulation test should be entered in the respective template.
Indicate according to which test guideline the study was conducted. If no test guideline was explicitly followed, but the methodology used is equivalent or similar to a specific guideline, you can indicate so in the "Qualifier" subfield preceding the field "Guideline".
Copy this block of fields for specifying more than one guideline (e.g. US EPA in addition to OECD guideline).
Table E.170. Field Descriptions
| Qualifier |
Select appropriate qualifier, i.e. - "according to" (if a given test guideline was followed); - "equivalent or similar to" (if no test guideline was explicitly followed, but the methodology is equivalent or similar to a specific guideline); - "no guideline followed" (if none of above qualifiers apply. If so, fill in field "Principles of method if other than guideline"); - "no guideline available" (if so, fill in field "Principles of method if other than guideline"). - "no guideline required" (if so, fill in field "Principles of method if other than guideline"). |
| Guideline |
Select the applicable test guideline, e.g. "OECD Guideline xxx". If the test guideline used is not listed, choose "other guideline:" and specify the test guideline in the related text field. In this text field, you can also enter any remarks as applicable, particularly: - To include any other title of the test guideline draft used, a subtitle, another version or update number and the year of update (For instance, different titles and/or numbers may exist for a given EU test guideline.); - To indicate if a the study was performed prior to the adoption of the test guideline specified; - To indicate if the methodology used was based on an extension of the test guideline specified. |
| Deviations from guideline (Deviations) |
For robust study summaries or as requested by the regulatory programme, indicate if there are any deviations from the test guideline specified. If "yes" is selected, only briefly state relevant deviations in the supplementary remarks field (e.g. "other species used"); details should be described in the respective fields of the section MATERIALS AND METHODS. |
If no guideline was followed, include a description of the principles of the test protocol or estimated method used in the study. Details should be entered in appropriate distinct fields of section MATERIALS AND METHODS if available. Also provide a justification for using this method if appropriate.
If an estimation method was used (to be indicated in field "Test result type") state the equation(s) and/or computer software or other methods applied to calculate the value(s).
Indicate whether the study was conducted following Good Laboratory Practice or not. Select "yes (incl. certificate)" if a GLP certificate of a test facility is available. Select "yes" if a GLP compliance statement is available, but no information on a GLP certificate. You can give an explanation in the supplementary remarks field, e.g. for explaining why GLP was not complied with or for specifying which (national) GLP was followed.
Indicate if the test material used in the study is equivalent to the submission substance identity. If "yes" is selected, the corresponding identity is automatically entered in the subsequent block of fields "Test material identity".
If "no" is selected, identify the test material in the subsequent block of fields "Test material identity". In this case, also make sure that the information entered in field "Study result type" is consistent, i.e. "read-across from supporting substance (structural analogue or surrogate)".
NOTE: If a completed record is used for another submission, you may have to update both fields "Study result type" and "Test material equivalent to submission substance identity".
If the identity of the test material used for this study is not included in this block of fields automatically, indicate the identity for one or more appropriate identifiers, e.g. CAS number, CAS name, IUPAC name. Copy this block of fields as appropriate.
If another than the submission substance identity was selected erraneously, go back to field "Test material equivalent to submission substance identity" and select "yes". This will prompt automatic entry of the respective identifiers.
Table E.171. Field Descriptions
| Identifier |
Select an appropriate identifier from drop-down list, e.g. "CAS number". Use "Other:" and specify, if identity according to a standard identifier is not known or if an additional chemical name or number is provided. |
| Identity |
Select the corresponding substance identity from drop-down list or enter manually if the identity is not available from the list or if no list is provided for the type of identifier selected. |
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Note that any information that can be claimed confidential should be included in the subsequent field "Confidential details on test material".
Explanations:
- Name of test material (as cited in study report): only if different from any other identifiers provided in the preceding fields.
- Molecular formula (if other than submission substance): specify
- Molecular weight (if other than submission substance): specify
- Smiles notation (if other than submission substance): provide if available
- InChl (if other than submission substance): provide if available
- Structural formula attached as image file (if other than submission substance): see Fig.: only if different from submission substance. Indicate Fig. no. if a file is attached in field "Attached document", e.g. state "see Fig. 1".
- Substance type: indicate whether pure active substance, technical product, formulation or other.
- Physical state: indicate "gas", "solid" or "liquid" only if different from submission substance or if substance can occur in different physical states.
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Isomers composition: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: provide if available
- Expiration date of the lot/batch: provide if available
- Radiochemical purity (if radiolabelling): specify if applicable
- Specific activity (if radiolabelling): specify if applicable
- Locations of the label (if radiolabelling): specify if applicable
- Expiration date of radiochemical substance (if radiolabelling): specify if applicable
- Storage condition of test substance: specify if applicable
- Stability under test conditions: indicate if available
Enter any confidential information on the test material in this separate field. Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Explanations:
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: : provide if available
- Expiration date of the lot/batch: : provide if available
- Isomers composition: specify if applicable
ONLY if another substance, i.e. analogue or surrogate (e.g. degradation/transformation product) was used as test material, enter any relevant details on the properties of this substance that may possibly affect the test performance, particularly physico-chemical properties.
Use freetext template and delete/add elements as appropriate.
Note that the physico-chemical properties of the substance for which the submission is made should be recorded in the corresponding templates and therefore need not be repeated here. Nevertheless, the possible influence of any physico-chemical properties should be indicated / discussed in appropriate fields, particularly in fields "Details on study design" and "Test performance". This holds also true if any property of the substance is different in the test medium as compared to the data recorded in the section on physico-chemical properties, e.g. lower water solubility.
Indicate whether test was performed under aerobic or anaerobic conditions. Include any explanations in the supplementary remarks field as appropriate.
Select the inoculum used. As far as possible, indicate whether any activated sludge or sewage used was adapted or not. If the study report does not give clear information thereof, select "..... (adaptation not specified)", e.g. "sewage, domestic (adaptation not specified)". In this case, give further explanation in field "Details on inoculum", if any. In field "Rationale for reliability", discuss the impact of this reporting deficiency on the study results.
If no inoculum was added in the strictest sense, but natural water and/or sediment was used, indicate the corresponding test system, e.g. "natural water / sediment".
Note that any simulation tests should be recorded using the corresponding template.
Give details on inoculum as appropriate. Use freetext template and delete/add elements as appropriate.
Indicate duration of test in terms of contact time. If different test runs have different durations, enter lower and upper value in respective subfields.
Table E.172. Field Descriptions
| Duration (no label) |
Enter numeric value(s) (incl. qualifier(s) if any) according to following conventions: (i) Enter single value with no qualifier (left blank) or preceded by ">", ">=" or "ca." in first numeric field; (ii) Enter single value preceded by "<" or "<=" in second numeric field; (iii) Enter range in both numeric fields including qualifier(s) if any. |
| Unit (no label) | Select from drop-down list. |
Specify the initial test concentration applied. If different concentrations were used in different test runs, copy this block of fields accordingly. If a range of concentrations is reported, include the lower and upper values in the numeric range field.
If appropriate copy this block of fields for indicating different parameters the initial concentration is based on (e.g. COD and test substance).
Table E.173. Field Descriptions
| Initial concentration (Initial conc.) |
Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) |
Select from drop-down list. |
| Based on |
From drop-down list, select the parameter on which the initial concentration is based. |
Indicate the parameter used to measure biodegradation. Copy field for more than one parameter as appropriate. In supplementary remarks field, give relevant details on the method. For radiochemical measurement or test substance analysis use freetext template in field "Details on analytical methods".
If the amount of test material in the test solutions was monitored, enter any details on the analytical methods used. Use freetext template and delete/add elements as appropriate. Copy any subheading(s) under IDENTIFICATION AND QUANTIFICATION OF PARENT COMPOUND to include the respective information for transformation products.
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Indicate identity of reference substance(s) used and give details in the supplementary remarks field as appropriate. Repeat field for each substance.
In this field, you can enter any information on materials and methods, for which no distinct field is available, or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Describe relevant results from preliminary study performed, if any (e.g., adsorption of test material to the walls of the test container).
Report on any unusual observations during test or any other information affecting results. Give reasons for any rejection of the test results if applicable.
Note that any deviations from test procedure should be briefly stated in field "Deviations from guideline".
Indicate percentage of degradation of test substance including standard deviation at the end of the study period. Indicate the parameter the percentage is based on and the sampling time. Copy this block of fields for recording the percentage values for different parameters.
Note that the degradation at different sampling time points (raw data) should be recorded in below field "Details on results".
NOTE: BOD*100/COD results should be entered in the respective fields below.
Table E.174. Field Descriptions
| % degradation (%Degr.) |
Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Standard deviation (St. dev.) |
Enter numeric value. |
| Parameter |
From drop-down list, select the parameter on which the percentage is based. |
| Sampling time |
Enter numeric value. |
| Unit sampling time (no label) |
Select from drop-down list. |
| Remarks |
Enter any remarks related to the recorded values as appropriate. |
Record the degradation / elimination kinetics for the different types of test suspensions, i.e. percentage of degradation at different sampling time points.
For robust study summaries or as requested by the regulatory programme, include table(s) in the rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
In field "Attached background material", attach graph(s) with the full degradation or elimination curves for the test and reference substances, the lag phase, degradation phase, the 10-d window and slope.
For tests for ready biodegradability, in which oxygen consumption is used as analytical method (e.g. MITI method), a BOD curve against time should be attached. If requested by the regulatory programme, also include a table on the material (mass) balance of parent compound and transformation products and a table showing the percentage data for degradability measured as BOD, DOC and by specific chemical analysis (see predefined tables).
For BOD5 tests, copy this block of fields for entering BOD5 and COD values (or ranges if reported so) including the unit, and the ratio BOD5*100/COD (with no unit). If a BOD5/COD ratio is reported, multiply the original value by 100.
Include any raw data in field "Any other information on results incl. tables".
Table E.175. Field Descriptions
| Parameter (no label) |
Select from drop-down list: BOD5, COD or BOD5*100/COD. |
| Value (no label) |
Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) |
Select unit from drop-down list or leave empty if "BOD5*100/COD" was selected as parameter. |
Indicate whether the results with the reference substance(s) are valid.
In this field, you can enter any overall remarks or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
In this field, you can enter any overall remarks or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Attach any background document that cannot be inserted in any rich text editor field, particularly image files (e.g. an image of a structural formula).
Copy this block of fields for attaching more than one file.
Table E.176. Field Descriptions
| Attached document |
Upload file by clicking the upload icon. As appropriate, enter any additional information, e.g. language. The file name is displayed after uploading the document. |
| Remarks |
As appropriate, include remarks, e.g. a short description of the content of the attached document if the file name is not self-explanatory. |
If required, an electronic copy of the full study report can be attached as WORD, pdf or other document type, which will not be integrated in any report, but must be handled as separate files.
Note: In the export administration you can indicate whether the attached files should be included in the data export or not.
Indicate whether validity criteria given by test guideline have been fulfilled or not. Use supplementary remarks field for indicating the criteria and entering remarks. Clearly indicate if the criteria used are not consistent with those given by the test guideline.
Indicate overall interpretation of test results with respect to the possible biodegradability as given in the study report. For more detailed discussion of test results, use field "Conclusions".
If required by the respective national/regional programme, briefly summarise the relevant aspects of the study including the conclusions reached. If a specific format is prescribed, upload the respective freetext template if available from the drop-down list or copy it from the corresponding document.
Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
A Cross-reference to other study or other studies can be included which are considered relevant in the interpretation of the test results, e.g. for supporting the conclusion that an effect observed was not substance-related. Indicate the respective chapter(s) and record ID(s) and enter relevant explanatory text.
Such cross-references may be useful if it is considered relevant to discuss other results at the summary level of a single study. It should be noted that the overall appraisal of results from different studies is normally done in the hazard or risk assessment.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
In the following, the online help texts for all data entry fields provided with any Endpoint summary record for this IUCLID section are listed. As to whether and to what extent endpoint summaries should be prepared, refer to the relevant guidance for the respective chemical programme, e.g., the Technical Guidance Document (TGD) on preparing the Chemical Safety Report under REACH in its most recent version.
For technical guidance on how to manage Endpoint summary records, see How to manage Endpoint summary records in sections 4 - 10, respectively. For details on data types, see What data types are available for input fields and how are they used?.
Enter a full short description of the most relevant endpoint data. This includes in principle the summary information that is compiled e.g. in the OECD Full SIDS Summary format or other endpoint summary formats. Provide only the most relevant details, which could be, depending on the cases, the test guideline used, test organism and exposure duration. Normally no reliability score needs to be indicated as it can be assumed that the studies identified are valid (reliability score 1 or 2). If this is not the case (for instance if the reliability of a published study cannot be assigned or if several less valid studies are used for a weight of evidence analysis), the reliability indicators should be specified.
Also several key studies can be referenced as applicable. However, the characterisation of the endpoint data should be kept as concise as possible. Examples of what could be entered here are as follows:
- Melting point: 54.6-55.8 °C at 1,013 hPa
- Water solubility: completely miscible
- pH: 6.9 (80 mg/l water) at 20°C (OECD TG105)
- Oxidation reduction potential: no data available
- Phototransformation in air: T1/2 = 9.32 x 10-2 yr (sensitizer: OH radical) (AOP Win v 1.86)
- Biodegradation in water: screening tests: Not readily biodegradable: 0 - 8% (BOD) in 28 days, 0 - 1% (HPLC) in 28 days (OECD TG 301C)
- Short-term toxicity to fish:
LC50 (96h) < 100 mg/l for Pimephales promelas (OECD TG 203)
LC50 (48h) = 3.2 mg/l for Oryzias latipes (OECD TG 203)
- Acute toxicity:
Dermal: LD50: > 2,000 mg/kg for rat (limit test)
- Genetic toxicity:
In vitro:
Gene mutation (Bacterial reverse mutation assay / Ames test): S. typhimurium TA 100: positive with and without metabolic activation; TA 1535: positive without metabolic activation (equivalent to OECD TG 471)
The field(s) under this heading (if provided) can be optionally completed in order to specify the key parameter(s) that may subsequently be used in the chemical safety assessment, classification and labelling or other. In order to enable the use of any specific software, only a minimum number of structured and hence, searchable fields are provided, in many cases only one.
Key parameters are intended to condense the data summarised in field "Full short description of relevant endpoint data" to one single numeric value or concluding remark (e.g. negative / positive) chosen from a drop-down list. Where a numeric field is provided, only a clear value can be entered, that is, no range and no less than or greater than qualifiers. Conversion to a predefined unit as indicated in the field label (e.g. mg/L) may be required.
If the key value is no clear number, but a range or preceded by <, <=, >, or >=, you may either leave this field empty or make up a value you consider most appropriate for the intended subsequent purpose. The rationale for any user-derived values should be described in field "Discussion" for the sake of transparency. Some non-exhaustive examples of user-derived parameters are as follows:
- Aquatic short-term L(E)C50 >100 mg/L (e.g. because only tested up to water solubility of the substance): it may be appropriate to assume 100 mg/L as worst case value.
- Aquatic long-term LOEC 1 mg/L (>10 and <20% effect): calculate NOEC as LOEC/2 and enter 0.5 mg/L in the field for NOEC.
- Acute oral LD50 >2000 mg/kg b.w. (because no LD50 was achieved in a limit test): enter 2000 mg/kg b.w.
In this rich text field, describe the assessment you have made for the given endpoint. Provide the rationale for the choice of the key study(ies) and the choice of the key parameter that, according to your judgement, characterise the endpoint. This includes a discussion of the key information identified and in some instances of studies which are considered to be unreliable, but give critical results. A discussion as to why they were discarded in favour of other studies should then be included. Vice versa, a weight of evidence analysis based on less reliable data or use of published data, the reliability of which cannot be judged because of limited reporting, should be justified.
If several studies were identified to be relevant for the assessment, discuss possible reasons for differing results if any, e.g. differences in purity / impurities of the test substance used, differences in the methods and test conditions, etc.
In the following, the online help texts for all data entry fields provided with any Endpoint study record for this IUCLID section are listed. For sections 4 to 10, these guidance notes are completely based on the so-called OECD Harmonised Templates (see Rationale behind IUCLID Endpoint Study Records - OECD harmonised templates in chapter chapter D.4.7.1 What is an Endpoint study record?)
IUCLID per se does not prescribe how detailed the study summaries should be recorded. Refer to the relevant guidance for the respective chemical regulatory programme thereof.
For technical guidance on how to manage Endpoint study records, see chapter D.4.7 How to manage Endpoint study records in sections 4 - 13. For details on data types, see chapter D.4.5 What data types are available for input fields and how are they used?
Under this main heading, fields are subsumed for identifying the purpose of the record (e.g., "key study"), the type of result (e.g., "experimental study"), data waiving indication (if any), reliability indication, and flags for indicating the regulatory purpose envisaged and/or any confidentiality restrictions. This kind of data characterise the relevance of a study summary and are therefore displayed on top of each Endpoint Study Record. For detailed guidance, refer to chapter D.4.7.7.1 Administrative data.
Indicate the bibliographic reference of the study report or publication the study summary is based on. Always enter the primary reference in the first block of fields (i.e. Sort no. = 1), if there are more than one reference to be cited. Copy this block of fields for specifying any other references related to this record (e.g. report of a preliminary study or other documentation). If results of a study report have been published, indicate the full citation of that publication(s) in addition to the reference of the original study.
Table E.177. Field Descriptions
| Reference type | Indicate the type of reference, e.g. "Study report" or "Publication". Select "Other company data" to characterise any unpublished information from a company other than a study report. Select "Grey literature" for any other unpublished information or "other:" and specify. |
| Author(s) (or transferred reference) (Author) | For ease of sorting and searchability use following convention: Surname, Initial (Example 1: White D, Ruehl KJ, Borman SA & Little J. Example 2: Hartley M & Murray W (avoid unnecessary full-stops, commas)). If no individuals are cited as authors, enter name of company or organisation or "Anon." as appropriate. Note that the complete bibliographic reference may appear in this field after migration of unstructured data from existing databases. |
| Year | Enter year of study report or publication. For a study report this field should be completed to include it in any searches, regardless of whether the complete date is given in field "Report date". |
| Title | Include the title of the report. For publications, include the title of the article of a journal or article/chapter of a book (e.g. handbook). |
| Bibliographic source | Not relevant for any study report. For publications or any other literature source (grey literature) specify the following type of information: (i) Title of scientific journal or book (e.g. if handbook); (ii) Volume of journal; (iii) Editor, publisher, place of publication for books or articles in books; (iv) Pagination. Example 1 (journal): J. Agric. Food Chem. 38: 215-227 Example 2 (handbook): In: Lyman WJ (ed.) Handbook of chemical property estimation methods. Environmental behavior of organic compounds. McGraw-Hill Book Company 15.1-15.34, New York. |
| Testing laboratory | Either manually enter the name of the testing laboratory or select it from the picklist. In either case, editing is possible. |
| Report no. | Specify the report number allocated by the testing laboratory. Note that any company-specific study number should be included in the respective field. |
| Owner company | Either manually enter the identity of the company who owns the data or select it from the picklist. In either case, editing is possible. |
| Company study no. | Specify any company study no. if there is such a number and if it is different from the report no. of the testing laboratory. Otherwise leave field empty. |
| Report date | Specify the complete date of the study report, e.g. "2005-05-12" for 12 May 2005. Note that subfield "Year" should be completed in any case for sorting and searching purposes. |
Select appropriate indication for data access. Enter "Not applicable" if the summary consists of information that is commonly accessible such as guidance on safe use.
Indicate as appropriate. Note: "yes" should be selected only if "Data submitter is data owner" or "Data submitter has Letter of Access". Options "yes, but willing to share" or "yes, but not willing to share" may be relevant for specific regulatory programmes where the submitter is requested to indicate whether he is willing to share studies (e.g. with vertebrates).
In the supplementary remarks field, include an explanation as appropriate, i.e. justification for denial of sharing the corresponding study or refer to a document attached that provides justification (e.g. "for justification see attached document X")
A cross-reference can be included to indicate that the same study is recorded in another record. Indicate the respective chapter and record ID and enter relevant explanatory text. This may be useful if specific endpoints of a given study are described in another chapter (e.g. results on reproduction toxicity in case of a combined repeated dose / reproduction toxicity study) or if more than one experiment is described by the same study report, but included in separate records.
Check with the relevant guidance document whether all the methodology details must be repeated or whether a cross-reference to the same study in another chapter may suffice.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
Indicate according to which test guideline the study was conducted. If no test guideline was explicitly followed, but the methodology used is equivalent or similar to a specific guideline, you can indicate so in the "Qualifier" subfield preceding the field "Guideline".
Copy this block of fields for specifying more than one guideline (e.g. US EPA in addition to OECD guideline).
Table E.178. Field Descriptions
| Qualifier | Select appropriate qualifier, i.e. - "according to" (if a given test guideline was followed); - "equivalent or similar to" (if no test guideline was explicitly followed, but the methodology is equivalent or similar to a specific guideline); - "no guideline followed" (if none of above qualifiers apply. If so, fill in field "Principles of method if other than guideline"); - "no guideline available" (if so, fill in field "Principles of method if other than guideline"). - "no guideline required" (if so, fill in field "Principles of method if other than guideline"). |
| Guideline | Select the applicable test guideline, e.g. "OECD Guideline xxx". If the test guideline used is not listed, choose "other guideline:" and specify the test guideline in the related text field. In this text field, you can also enter any remarks as applicable, particularly: - To include any other title of the test guideline draft used, a subtitle, another version or update number and the year of update (For instance, different titles and/or numbers may exist for a given EU test guideline.); - To indicate if a the study was performed prior to the adoption of the test guideline specified; - To indicate if the methodology used was based on an extension of the test guideline specified. |
| Deviations from guideline (Deviations) | For robust study summaries or as requested by the regulatory programme, indicate if there are any deviations from the test guideline specified. If "yes" is selected, only briefly state relevant deviations in the supplementary remarks field (e.g. "other species used"); details should be described in the respective fields of the section MATERIALS AND METHODS. |
If no guideline was followed, include a description of the principles of the test protocol or estimated method used in the study. Details should be entered in appropriate distinct fields of section MATERIALS AND METHODS if available. Also provide a justification for using this method if appropriate.
If an estimation method was used (to be indicated in field "Test result type") state the equation(s) and/or computer software or other methods applied to calculate the value(s).
Indicate whether the study was conducted following Good Laboratory Practice or not. Select "yes (incl. certificate)" if a GLP certificate of a test facility is available. Select "yes" if a GLP compliance statement is available, but no information on a GLP certificate. You can give an explanation in the supplementary remarks field, e.g. for explaining why GLP was not complied with or for specifying which (national) GLP was followed.
Indicate if the test material used in the study is equivalent to the submission substance identity. If "yes" is selected, the corresponding identity is automatically entered in the subsequent block of fields "Test material identity".
If "no" is selected, identify the test material in the subsequent block of fields "Test material identity". In this case, also make sure that the information entered in field "Study result type" is consistent, i.e. "read-across from supporting substance (structural analogue or surrogate)".
NOTE: If a completed record is used for another submission, you may have to update both fields "Study result type" and "Test material equivalent to submission substance identity".
If the identity of the test material used for this study is not included in this block of fields automatically, indicate the identity for one or more appropriate identifiers, e.g. CAS number, CAS name, IUPAC name. Copy this block of fields as appropriate.
If another than the submission substance identity was selected erraneously, go back to field "Test material equivalent to submission substance identity" and select "yes". This will prompt automatic entry of the respective identifiers.
Table E.179. Field Descriptions
| Identifier | Select an appropriate identifier from drop-down list, e.g. "CAS number". Use "Other:" and specify, if identity according to a standard identifier is not known or if an additional chemical name or number is provided. |
| Identity | Select the corresponding substance identity from drop-down list or enter manually if the identity is not available from the list or if no list is provided for the type of identifier selected. |
Indicate if labelled or non-labelled test material was used. Details on labelled material to be described in field "Details on test material".
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Note that any information that can be claimed confidential should be included in the subsequent field "Confidential details on test material".
Explanations:
- Name of test material (as cited in study report): only if different from any other identifiers provided in the preceding fields.
- Molecular formula (if other than submission substance): specify
- Molecular weight (if other than submission substance): specify
- Smiles notation (if other than submission substance): provide if available
- InChl (if other than submission substance): provide if available
- Structural formula attached as image file (if other than submission substance): see Fig.: only if different from submission substance. Indicate Fig. no. if a file is attached in field "Attached document", e.g. state "see Fig. 1".
- Substance type: indicate whether pure active substance, technical product, formulation or other.
- Physical state: indicate "gas", "solid" or "liquid" only if different from submission substance or if substance can occur in different physical states.
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Isomers composition: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: provide if available
- Expiration date of the lot/batch: provide if available
- Radiochemical purity (if radiolabelling): specify if applicable
- Specific activity (if radiolabelling): specify if applicable
- Locations of the label (if radiolabelling): specify if applicable
- Expiration date of radiochemical substance (if radiolabelling): specify if applicable
- Storage condition of test substance: specify if applicable
- Stability under test conditions: indicate if available
Enter any confidential information on the test material in this separate field. Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Explanations:
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: : provide if available
- Expiration date of the lot/batch: : provide if available
- Isomers composition: specify if applicable
ONLY if another substance, i.e. analogue or surrogate (e.g. degradation/transformation product) was used as test material, enter any relevant details on the properties of this substance that may possibly affect the test performance, particularly physico-chemical properties.
Use freetext template and delete/add elements as appropriate.
Note that the physico-chemical properties of the substance for which the submission is made should be recorded in the corresponding templates and therefore need not be repeated here. Nevertheless, the possible influence of any physico-chemical properties should be indicated / discussed in appropriate fields, particularly in fields "Details on study design" and "Test performance". This holds also true if any property of the substance is different in the test medium as compared to the data recorded in the section on physico-chemical properties, e.g. lower water solubility.
Indicate whether test was performed under aerobic or anaerobic conditions. Select "aerobic/anaerobic" if both oxygen conditions occur as in water/sediment studies. Include any explanations in the supplementary remarks field as appropriate.
Select the inoculum used. As far as possible, indicate whether any activated sludge or sewage used was adapted or not. If the study report does not give clear information thereof, select "..... (adaptation not specified)", e.g. "sewage, domestic (adaptation not specified)". In this case, give further explanation in field "Details on inoculum", if any. In field "Rationale for reliability", discuss the impact of this reporting deficiency on the study results.
If no inoculum was added in the strictest sense, but natural water and/or sediment was used, indicate the corresponding test system, e.g. "natural water / sediment".
Note that any simulation tests should be recorded using the corresponding template.
Give details on source and properties of surface water used as inoculum if applicable. Use freetext template and delete/add elements as appropriate.
Give details on source and properties of sediment used as inoculum if applicable. Use freetext template and delete/add elements as appropriate.
Give details on any other inoculum, e.g. activated sludge if applicable. Use freetext template and delete/add elements as appropriate.
Indicate duration of test in terms of contact time. If different test runs have different durations, enter lower and upper value in respective subfields.
Table E.180. Field Descriptions
| Duration (no label) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) | Select from drop-down list. |
Specify the initial test concentration applied. If different concentrations were used in different test runs, copy this block of fields accordingly. If a range of concentrations is reported, include the lower and upper values in the numeric range field.
If appropriate copy this block of fields for indicating different parameters the initial concentration is based on (e.g. COD and test substance).
Table E.181. Field Descriptions
| Initial concentration (Initial conc.) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) | Select from drop-down list. |
| Based on | From drop-down list, select the parameter on which the initial concentration is based. |
Indicate the parameter used to measure biodegradation. Copy field for more than one parameter as appropriate. In supplementary remarks field, give relevant details on the method. For radiochemical measurement or test substance analysis use freetext template in field "Details on analytical methods".
If the amount of test material in the test solutions was monitored, enter any details on the analytical methods used. Use freetext template and delete/add elements as appropriate. Copy any subheading(s) under IDENTIFICATION AND QUANTIFICATION OF PARENT COMPOUND to include the respective information for transformation products and/or non-extractable residues.
Specify methods for water and sediment if applicable.
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Indicate identity of reference substance(s) used and give details in the supplementary remarks field as appropriate. Repeat field for each substance.
In this field, you can enter any information on materials and methods, for which no distinct field is available, or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Report on any unusual observations during test or any other information affecting results. Give reasons for any rejection of the test results if applicable.
Note that any deviations from test procedure should be briefly stated in field "Deviations from guideline".
If applicable, indicate mean total recovery of test material as percentage of applied amount in water and/or sediment +/- standard deviation.
Table E.182. Field Descriptions
| Mean total recovery in water (% in water) | Enter numeric value. |
| Standard deviation (St. dev.) | Enter numeric value. |
| Mean total recovery in sediment (% in sediment) | Enter numeric value. |
| Standard deviation (St. dev.) | Enter numeric value. |
| Remarks | Enter any remarks related to the recorded values as appropriate. |
Indicate percentage of degradation of test substance including standard deviation at the end of the study period. Indicate the parameter the percentage is based on and the sampling time. Copy this block of fields for recording the percentage values for different parameters.
Note that the degradation at different sampling time points (raw data) should be recorded in below field "Details on results".
Table E.183. Field Descriptions
| % degradation (%Degr.) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Standard deviation (St. dev.) | Enter numeric value. |
| Parameter | From drop-down list, select the parameter on which the percentage is based. |
| Sampling time | Enter numeric value. |
| Unit sampling time (no label) | Select from drop-down list. |
| Remarks | Enter any remarks related to the recorded values as appropriate. |
Include value (or range if reported so) of half-life and indicate the type of half-life (For first order kinetics DT50 = half-life). For water-sediment systems repeat this block of fields for each compartment.
Table E.184. Field Descriptions
| Compartment | Select from drop-down list. |
| Half-life | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) | Select from drop-down list. |
| St. dev. | Enter numeric value. |
| Type of half-life (Type) | Select from drop-down list. |
| Remarks (e.g. regression equation, r², DT90) (Remarks (e.g. regr. equ., r², DT90)) | For robust study summaries or as requested by the regulatory programme, provide the regression equation, r² , DT90 (with unit) and/or any relevant remarks. |
Include any other relevant kinetic parameters if applicable. Select the respective item from the picklist and include the value in the associated remarks field. Copy this field for each parameter to be entered.
Indicate whether transformation products occurred. If yes, provide the identified transformation products in following block of fields. Any further details can be entered in field "Any other information on results incl. tables".
Indicate the identity of the transformation products using an appropriate identifier, e.g. CAS number, CAS name, IUPAC name. Copy this block of fields for each relevant substance.
Any further details on transformation products can be provided in field "Any other information on materials and methods incl. tables".
Table E.185. Field Descriptions
| No. | For easier distinction select a consecutive number for each transformation product from drop-down list if more than one transformation product is entered. If the same substance is identified by more than one identifiers (e.g. by CAS name and Common name), make sure that the same number is allocated to these entries. |
| Identifier | Select an appropriate identifier from drop-down list, e.g. "CAS number". Use "Other:" if identity according to a standard identifier is not known. Specify if another identifier is provided (e.g. "Other: xxx"). |
| Identity | Select the corresponding substance identity from drop-down list or enter manually if the identity is not available from the list or if no list is provided for the type of identifier selected. |
Indicate any relevant supplementary information on transformation products. Use freetext template and delete/add items as appropriate. If useful attach a figure in the corresponding field.
Indicate whether evaporation of the parent compound occurred or not. Include any explanations in field "Details on results" as appropriate.
Indicate whether volatile metabolites were found or not. Include any explanations in field "Details on results" as appropriate.
Indicate whether residues were found or not. Include any explanations in field "Details on results" as appropriate.
Indicate any further relevant details of test results. Use freetext template and delete/add elements as appropriate. As appropriate or requested by the regulatory programme include table(s) with raw data in the rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
In field "Attached background material", attach graph(s) with the full degradation or elimination curves.
TEST CONDITIONS: If the test conditions were not maintained, describe any anomalies or problems encountered.
MAJOR / MINOR TRANSFORMATION PRODUCTS: Indicate concentration ranges of the transformation products specified in the defined field "Identity of transformation products" or specify if no major transformation products were detected. Tabulate comprehensive data and refer to respective table no. (use predefined table if any) or other appropriate table.
STERILE TREATMENTS: If used, report the transformation of the parent, and compare the results with those of the non-sterile treatments:
SUPPLEMENTARY EXPERIMENT: Briefly describe the results of the supplementary experiment, if any.
Indicate whether the results with the reference substance(s) are valid.
In this field, you can enter any other remarks on results. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: Both the "Materials and methods" section and "Results" section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
In this field, you can enter any overall remarks or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Attach any background document that cannot be inserted in any rich text editor field, particularly image files (e.g. an image of a structural formula).
Copy this block of fields for attaching more than one file.
Table E.186. Field Descriptions
| Attached document | Upload file by clicking the upload icon. As appropriate, enter any additional information, e.g. language. The file name is displayed after uploading the document. |
| Remarks | As appropriate, include remarks, e.g. a short description of the content of the attached document if the file name is not self-explanatory. |
If required, an electronic copy of the full study report can be attached as WORD, pdf or other document type, which will not be integrated in any report, but must be handled as separate files.
Note: In the export administration you can indicate whether the attached files should be included in the data export or not.
Indicate whether validity criteria given by test guideline have been fulfilled or not. Use supplementary remarks field for indicating the criteria and entering remarks. Clearly indicate if the criteria used are not consistent with those given by the test guideline. If so, give justification in field "Rationale for reliability incl. deficiences" as to why this study summary is considered reliable.
If required by the respective national/regional programme, briefly summarise the relevant aspects of the study including the conclusions reached. If a specific format is prescribed, upload the respective freetext template if available from the drop-down list or copy it from the corresponding document.
Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
A Cross-reference to other study or other studies can be included which are considered relevant in the interpretation of the test results, e.g. for supporting the conclusion that an effect observed was not substance-related. Indicate the respective chapter(s) and record ID(s) and enter relevant explanatory text.
Such cross-references may be useful if it is considered relevant to discuss other results at the summary level of a single study. It should be noted that the overall appraisal of results from different studies is normally done in the hazard or risk assessment.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
In the following, the online help texts for all data entry fields provided with any Endpoint summary record for this IUCLID section are listed. As to whether and to what extent endpoint summaries should be prepared, refer to the relevant guidance for the respective chemical programme, e.g., the Technical Guidance Document (TGD) on preparing the Chemical Safety Report under REACH in its most recent version.
For technical guidance on how to manage Endpoint summary records, see How to manage Endpoint summary records in sections 4 - 10, respectively. For details on data types, see What data types are available for input fields and how are they used?.
Enter a full short description of the most relevant endpoint data. This includes in principle the summary information that is compiled e.g. in the OECD Full SIDS Summary format or other endpoint summary formats. Provide only the most relevant details, which could be, depending on the cases, the test guideline used, test organism and exposure duration. Normally no reliability score needs to be indicated as it can be assumed that the studies identified are valid (reliability score 1 or 2). If this is not the case (for instance if the reliability of a published study cannot be assigned or if several less valid studies are used for a weight of evidence analysis), the reliability indicators should be specified.
Also several key studies can be referenced as applicable. However, the characterisation of the endpoint data should be kept as concise as possible. Examples of what could be entered here are as follows:
- Melting point: 54.6-55.8 °C at 1,013 hPa
- Water solubility: completely miscible
- pH: 6.9 (80 mg/l water) at 20°C (OECD TG105)
- Oxidation reduction potential: no data available
- Phototransformation in air: T1/2 = 9.32 x 10-2 yr (sensitizer: OH radical) (AOP Win v 1.86)
- Biodegradation in water: screening tests: Not readily biodegradable: 0 - 8% (BOD) in 28 days, 0 - 1% (HPLC) in 28 days (OECD TG 301C)
- Short-term toxicity to fish:
LC50 (96h) < 100 mg/l for Pimephales promelas (OECD TG 203)
LC50 (48h) = 3.2 mg/l for Oryzias latipes (OECD TG 203)
- Acute toxicity:
Dermal: LD50: > 2,000 mg/kg for rat (limit test)
- Genetic toxicity:
In vitro:
Gene mutation (Bacterial reverse mutation assay / Ames test): S. typhimurium TA 100: positive with and without metabolic activation; TA 1535: positive without metabolic activation (equivalent to OECD TG 471)
The field(s) under this heading (if provided) can be optionally completed in order to specify the key parameter(s) that may subsequently be used in the chemical safety assessment, classification and labelling or other. In order to enable the use of any specific software, only a minimum number of structured and hence, searchable fields are provided, in many cases only one.
Key parameters are intended to condense the data summarised in field "Full short description of relevant endpoint data" to one single numeric value or concluding remark (e.g. negative / positive) chosen from a drop-down list. Where a numeric field is provided, only a clear value can be entered, that is, no range and no less than or greater than qualifiers. Conversion to a predefined unit as indicated in the field label (e.g. mg/L) may be required.
If the key value is no clear number, but a range or preceded by <, <=, >, or >=, you may either leave this field empty or make up a value you consider most appropriate for the intended subsequent purpose. The rationale for any user-derived values should be described in field "Discussion" for the sake of transparency. Some non-exhaustive examples of user-derived parameters are as follows:
- Aquatic short-term L(E)C50 >100 mg/L (e.g. because only tested up to water solubility of the substance): it may be appropriate to assume 100 mg/L as worst case value.
- Aquatic long-term LOEC 1 mg/L (>10 and <20% effect): calculate NOEC as LOEC/2 and enter 0.5 mg/L in the field for NOEC.
- Acute oral LD50 >2000 mg/kg b.w. (because no LD50 was achieved in a limit test): enter 2000 mg/kg b.w.
In this rich text field, describe the assessment you have made for the given endpoint. Provide the rationale for the choice of the key study(ies) and the choice of the key parameter that, according to your judgement, characterise the endpoint. This includes a discussion of the key information identified and in some instances of studies which are considered to be unreliable, but give critical results. A discussion as to why they were discarded in favour of other studies should then be included. Vice versa, a weight of evidence analysis based on less reliable data or use of published data, the reliability of which cannot be judged because of limited reporting, should be justified.
If several studies were identified to be relevant for the assessment, discuss possible reasons for differing results if any, e.g. differences in purity / impurities of the test substance used, differences in the methods and test conditions, etc.
In the following, the online help texts for all data entry fields provided with any Endpoint study record for this IUCLID section are listed. For sections 4 to 10, these guidance notes are completely based on the so-called OECD Harmonised Templates (see Rationale behind IUCLID Endpoint Study Records - OECD harmonised templates in chapter chapter D.4.7.1 What is an Endpoint study record?)
IUCLID per se does not prescribe how detailed the study summaries should be recorded. Refer to the relevant guidance for the respective chemical regulatory programme thereof.
For technical guidance on how to manage Endpoint study records, see chapter D.4.7 How to manage Endpoint study records in sections 4 - 13. For details on data types, see chapter D.4.5 What data types are available for input fields and how are they used?
Under this main heading, fields are subsumed for identifying the purpose of the record (e.g., "key study"), the type of result (e.g., "experimental study"), data waiving indication (if any), reliability indication, and flags for indicating the regulatory purpose envisaged and/or any confidentiality restrictions. This kind of data characterise the relevance of a study summary and are therefore displayed on top of each Endpoint Study Record. For detailed guidance, refer to chapter D.4.7.7.1 Administrative data.
Indicate the bibliographic reference of the study report or publication the study summary is based on. Always enter the primary reference in the first block of fields (i.e. Sort no. = 1), if there are more than one reference to be cited. Copy this block of fields for specifying any other references related to this record (e.g. report of a preliminary study or other documentation). If results of a study report have been published, indicate the full citation of that publication(s) in addition to the reference of the original study.
Table E.187. Field Descriptions
| Reference type | Indicate the type of reference, e.g. "Study report" or "Publication". Select "Other company data" to characterise any unpublished information from a company other than a study report. Select "Grey literature" for any other unpublished information or "other:" and specify. |
| Author(s) (or transferred reference) (Author) | For ease of sorting and searchability use following convention: Surname, Initial (Example 1: White D, Ruehl KJ, Borman SA & Little J. Example 2: Hartley M & Murray W (avoid unnecessary full-stops, commas)). If no individuals are cited as authors, enter name of company or organisation or "Anon." as appropriate. Note that the complete bibliographic reference may appear in this field after migration of unstructured data from existing databases. |
| Year | Enter year of study report or publication. For a study report this field should be completed to include it in any searches, regardless of whether the complete date is given in field "Report date". |
| Title | Include the title of the report. For publications, include the title of the article of a journal or article/chapter of a book (e.g. handbook). |
| Bibliographic source | Not relevant for any study report. For publications or any other literature source (grey literature) specify the following type of information: (i) Title of scientific journal or book (e.g. if handbook); (ii) Volume of journal; (iii) Editor, publisher, place of publication for books or articles in books; (iv) Pagination. Example 1 (journal): J. Agric. Food Chem. 38: 215-227 Example 2 (handbook): In: Lyman WJ (ed.) Handbook of chemical property estimation methods. Environmental behavior of organic compounds. McGraw-Hill Book Company 15.1-15.34, New York. |
| Testing laboratory | Either manually enter the name of the testing laboratory or select it from the picklist. In either case, editing is possible. |
| Report no. | Specify the report number allocated by the testing laboratory. Note that any company-specific study number should be included in the respective field. |
| Owner company | Either manually enter the identity of the company who owns the data or select it from the picklist. In either case, editing is possible. |
| Company study no. | Specify any company study no. if there is such a number and if it is different from the report no. of the testing laboratory. Otherwise leave field empty. |
| Report date | Specify the complete date of the study report, e.g. "2005-05-12" for 12 May 2005. Note that subfield "Year" should be completed in any case for sorting and searching purposes. |
Select appropriate indication for data access. Enter "Not applicable" if the summary consists of information that is commonly accessible such as guidance on safe use.
Indicate as appropriate. Note: "yes" should be selected only if "Data submitter is data owner" or "Data submitter has Letter of Access". Options "yes, but willing to share" or "yes, but not willing to share" may be relevant for specific regulatory programmes where the submitter is requested to indicate whether he is willing to share studies (e.g. with vertebrates).
In the supplementary remarks field, include an explanation as appropriate, i.e. justification for denial of sharing the corresponding study or refer to a document attached that provides justification (e.g. "for justification see attached document X")
A cross-reference can be included to indicate that the same study is recorded in another record. Indicate the respective chapter and record ID and enter relevant explanatory text. This may be useful if specific endpoints of a given study are described in another chapter (e.g. results on reproduction toxicity in case of a combined repeated dose / reproduction toxicity study) or if more than one experiment is described by the same study report, but included in separate records.
Check with the relevant guidance document whether all the methodology details must be repeated or whether a cross-reference to the same study in another chapter may suffice.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
Indicate according to which test guideline the study was conducted. If no test guideline was explicitly followed, but the methodology used is equivalent or similar to a specific guideline, you can indicate so in the "Qualifier" subfield preceding the field "Guideline".
Copy this block of fields for specifying more than one guideline (e.g. US EPA in addition to OECD guideline).
Table E.188. Field Descriptions
| Qualifier | Select appropriate qualifier, i.e. - "according to" (if a given test guideline was followed); - "equivalent or similar to" (if no test guideline was explicitly followed, but the methodology is equivalent or similar to a specific guideline); - "no guideline followed" (if none of above qualifiers apply. If so, fill in field "Principles of method if other than guideline"); - "no guideline available" (if so, fill in field "Principles of method if other than guideline"). - "no guideline required" (if so, fill in field "Principles of method if other than guideline"). |
| Guideline | Select the applicable test guideline, e.g. "OECD Guideline xxx". If the test guideline used is not listed, choose "other guideline:" and specify the test guideline in the related text field. In this text field, you can also enter any remarks as applicable, particularly: - To include any other title of the test guideline draft used, a subtitle, another version or update number and the year of update (For instance, different titles and/or numbers may exist for a given EU test guideline.); - To indicate if a the study was performed prior to the adoption of the test guideline specified; - To indicate if the methodology used was based on an extension of the test guideline specified. |
| Deviations from guideline (Deviations) | For robust study summaries or as requested by the regulatory programme, indicate if there are any deviations from the test guideline specified. If "yes" is selected, only briefly state relevant deviations in the supplementary remarks field (e.g. "other species used"); details should be described in the respective fields of the section MATERIALS AND METHODS. |
If no guideline was followed, include a description of the principles of the test protocol or estimated method used in the study. Details should be entered in appropriate distinct fields of section MATERIALS AND METHODS if available. Also provide a justification for using this method if appropriate.
If an estimation method was used (to be indicated in field "Test result type") state the equation(s) and/or computer software or other methods applied to calculate the value(s).
Indicate whether the study was conducted following Good Laboratory Practice or not. Select "yes (incl. certificate)" if a GLP certificate of a test facility is available. Select "yes" if a GLP compliance statement is available, but no information on a GLP certificate. You can give an explanation in the supplementary remarks field, e.g. for explaining why GLP was not complied with or for specifying which (national) GLP was followed.
Indicate if the test material used in the study is equivalent to the submission substance identity. If "yes" is selected, the corresponding identity is automatically entered in the subsequent block of fields "Test material identity".
If "no" is selected, identify the test material in the subsequent block of fields "Test material identity". In this case, also make sure that the information entered in field "Study result type" is consistent, i.e. "read-across from supporting substance (structural analogue or surrogate)".
NOTE: If a completed record is used for another submission, you may have to update both fields "Study result type" and "Test material equivalent to submission substance identity".
If the identity of the test material used for this study is not included in this block of fields automatically, indicate the identity for one or more appropriate identifiers, e.g. CAS number, CAS name, IUPAC name. Copy this block of fields as appropriate.
If another than the submission substance identity was selected erraneously, go back to field "Test material equivalent to submission substance identity" and select "yes". This will prompt automatic entry of the respective identifiers.
Table E.189. Field Descriptions
| Identifier | Select an appropriate identifier from drop-down list, e.g. "CAS number". Use "Other:" and specify, if identity according to a standard identifier is not known or if an additional chemical name or number is provided. |
| Identity | Select the corresponding substance identity from drop-down list or enter manually if the identity is not available from the list or if no list is provided for the type of identifier selected. |
Indicate if labelled or non-labelled test material was used. Details on labelled material to be described in field "Details on test material".
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Note that any information that can be claimed confidential should be included in the subsequent field "Confidential details on test material".
Explanations:
- Name of test material (as cited in study report): only if different from any other identifiers provided in the preceding fields.
- Molecular formula (if other than submission substance): specify
- Molecular weight (if other than submission substance): specify
- Smiles notation (if other than submission substance): provide if available
- InChl (if other than submission substance): provide if available
- Structural formula attached as image file (if other than submission substance): see Fig.: only if different from submission substance. Indicate Fig. no. if a file is attached in field "Attached document", e.g. state "see Fig. 1".
- Substance type: indicate whether pure active substance, technical product, formulation or other.
- Physical state: indicate "gas", "solid" or "liquid" only if different from submission substance or if substance can occur in different physical states.
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Isomers composition: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: provide if available
- Expiration date of the lot/batch: provide if available
- Radiochemical purity (if radiolabelling): specify if applicable
- Specific activity (if radiolabelling): specify if applicable
- Locations of the label (if radiolabelling): specify if applicable
- Expiration date of radiochemical substance (if radiolabelling): specify if applicable
- Storage condition of test substance: specify if applicable
- Stability under test conditions: indicate if available
Enter any confidential information on the test material in this separate field. Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Explanations:
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: : provide if available
- Expiration date of the lot/batch: : provide if available
- Isomers composition: specify if applicable
ONLY if another substance, i.e. analogue or surrogate (e.g. degradation/transformation product) was used as test material, enter any relevant details on the properties of this substance that may possibly affect the test performance, particularly physico-chemical properties.
Use freetext template and delete/add elements as appropriate.
Note that the physico-chemical properties of the substance for which the submission is made should be recorded in the corresponding templates and therefore need not be repeated here. Nevertheless, the possible influence of any physico-chemical properties should be indicated / discussed in appropriate fields, particularly in fields "Details on study design" and "Test performance". This holds also true if any property of the substance is different in the test medium as compared to the data recorded in the section on physico-chemical properties, e.g. lower water solubility.
Indicate whether test was performed under aerobic or anaerobic conditions. Include any explanations in the supplementary remarks field as appropriate.
Indicate the soil classification system used.
Repeat this block of fields for each different soil used as indicated by the Soil No. Enter soil type as cited in the study report and the respective soil properties.
Table E.191. Field Descriptions
| Soil No. | Select a consecutive soil number from drop-down list if more than one soil types were used. |
| Soil type | Select from drop-down list. |
| % clay (Clay %) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| % silt (Silt %) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| % sand (Sand %) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| % total organic carbon (Org. C %) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| pH | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Cation exchange capacity (CEC) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) | Select from drop-down list. |
| Bulk density (g/cm³) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
For each soil type, specifiy soil collection and storage and properties of the soil as far as not indicated in the defined fields.
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Specify duration of test in terms of contact time. Repeat block for each soil type. If different test runs have different durations, enter lower and upper value in respective subfields.
Table E.192. Field Descriptions
| Soil No. | Select a consecutive soil number from drop-down list if more than one soil types were used. |
| Duration | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) | Select from drop-down list. |
Specify initial test concentration applied in the test runs for each soil type, normally based on mg/kg soil dw. If appropriate repeat block of fields and indicate concentration(s) based on g/ha or kg/ha or other (to be specified).
Table E.193. Field Descriptions
| Soil No. | Select a consecutive soil number from drop-down list if more than one soil types were used. |
| Initial concentration (Initial conc.) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) | Select from drop-down list. |
| Based on | From drop-down list, select type of substance on which the initial concentration is based. |
Indicate the parameter used to measure biodegradation. Copy field for more than one parameter as appropriate. In supplementary remarks field, give relevant details on the method. For radiochemical measurement or test substance analysis use freetext template in field "Details on analytical methods".
If the amount of test material in the test solutions was monitored, enter any details on the analytical methods used. Use freetext template and delete/add elements as appropriate. Copy any subheading(s) under IDENTIFICATION AND QUANTIFICATION OF PARENT COMPOUND to include the respective information for transformation products and/or non-extractable residues.
For each soil type, indicate the environmental conditions during the test if available or assumed in the model, if estimated.
Table E.194. Field Descriptions
| Soil No. | Select a consecutive soil number from drop-down list if more than one soil types were used. |
| Temperature (Temp.) | Specifiy test temperature including mean and range values during test if available or temperature assumed in the model, if estimated. Use °C; convert other units and indicate original data in parentheses if applicable. |
| Humidity | Indicate soil humidity in % moisture content or g water/100g soil dry weight. |
| Microbial biomass | Indicate initial and final microbial biomass / microbial population of control and treated soil, if provided. Specify unit, e.g., mg biomass/100 g soil dry weight or µg C/g soil. |
Include Soil No. in parentheses if conditions were not identical for all soil types tested. Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
In this field, you can enter any information on materials and methods, for which no distinct field is available, or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
If applicable, indicate mean total recovery of test material as percentage of applied amount +/- standard deviation. Copy this block of fields for each soil type as appropriate.
For each soil type, indicate percentage of degradation of test substance including standard deviation at the end of the study period. Also indicate on what parameter the degradation rate is based on (e.g. "radiochemical measurement"). If required, copy block of fields to include values based on different parameters.
Table E.196. Field Descriptions
| Soil No. | Select a consecutive soil number from drop-down list if more than one soil types were used. |
| % degradation (%Degr.) | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| St. dev. | Enter numeric value. |
| Parameter | From drop-down list, select the parameter on which the percentage is based. |
| Sampling time | Enter numeric value. |
| Unit sampling time (no label) | Select from drop-down list. |
For each soil type, include value (or range if reported so) of half-life and indicate the type of half-life (For first order kinetics DT50 = half-life).
Table E.197. Field Descriptions
| Soil No. | Select a consecutive soil number from drop-down list if more than one soil types were used. |
| Half-life | Enter a numeric value or a range of numeric values according to following conventions: (i) In the first numeric field, enter a single value (Qualifier subfield left blank) or a value if preceded by ">", ">=" or "ca." (e.g. "20", "ca. 20", ">20"). (ii) In the second numeric field, enter a single value if preceded by "<" or "<=". (iii) Use both numeric fields and, as required, the lower and upper qualifier field to enter a range of numeric values (e.g. "2 - 8" or ">2 <8"). |
| Unit (no label) | Enter numeric value. |
| St. dev. | Enter numeric value. |
| Type of half-life (Type) | Indicate the type of half-life (For first order kinetics DT50 = half-life). |
| Remarks (e.g. regression equation, r², DT90) (Remarks (e.g. regr. equ., r², DT90)) | For robust study summaries or as requested by the regulatory programme, provide the regression equation, r² , DT90 (with unit) and/or any relevant remarks. |
Indicate whether transformation products occurred. If yes, provide the identified transformation products in following block of fields. Any further details can be entered in field "Any other information on results incl. tables".
Indicate the identity of the transformation products using an appropriate identifier, e.g. CAS number, CAS name, IUPAC name. Copy this block of fields for each relevant substance.
Any further details on transformation products can be provided in field "Any other information on materials and methods incl. tables".
Table E.198. Field Descriptions
| No. | For easier distinction select a consecutive number for each transformation product from drop-down list if more than one transformation product is entered. If the same substance is identified by more than one identifiers (e.g. by CAS name and Common name), make sure that the same number is allocated to these entries. |
| Identifier | Select an appropriate identifier from drop-down list, e.g. "CAS number". Use "Other:" if identity according to a standard identifier is not known. Specify if another identifier is provided (e.g. "Other: xxx"). |
| Identity | Select the corresponding substance identity from drop-down list or enter manually if the identity is not available from the list or if no list is provided for the type of identifier selected. |
Indicate any relevant supplementary information on transformation products. Use freetext template and delete/add items as appropriate. If useful attach a figure in the corresponding field.
Indicate whether evaporation of the parent compound occurred or not. Include any explanations in field "Details on results" as appropriate.
Indicate whether volatile metabolites were found or not. Include any explanations in field "Details on results" as appropriate.
Indicate whether residues were found or not. Include any explanations in field "Details on results" as appropriate.
Indicate any further relevant details of test results. Use freetext template and delete/add elements as appropriate. As appropriate or requested by the regulatory programme include table(s) with raw data in the rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
In field "Attached background material", attach graph(s) with the full degradation or elimination curves.
TEST CONDITIONS: If the test conditions were not maintained, describe any anomalies or problems encountered.
MAJOR / MINOR TRANSFORMATION PRODUCTS: Indicate concentration ranges of the transformation products specified in the defined field "Identity of transformation products" or specify if no major transformation products were detected. Tabulate comprehensive data and refer to respective table no. (use predefined table if any) or other appropriate table.
STERILE TREATMENTS: If used, report the transformation of the parent, and compare the results with those of the non-sterile treatments:
SUPPLEMENTARY EXPERIMENT: Briefly describe the results of the supplementary experiment, if any.
Indicate whether the results with the reference substance(s) are valid.
In this field, you can enter any other remarks on results. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: Both the "Materials and methods" section and "Results" section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
In this field, you can enter any overall remarks or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Attach any background document that cannot be inserted in any rich text editor field, particularly image files (e.g. an image of a structural formula).
Copy this block of fields for attaching more than one file.
Table E.199. Field Descriptions
| Attached document | Upload file by clicking the upload icon. As appropriate, enter any additional information, e.g. language. The file name is displayed after uploading the document. |
| Remarks | As appropriate, include remarks, e.g. a short description of the content of the attached document if the file name is not self-explanatory. |
If required, an electronic copy of the full study report can be attached as WORD, pdf or other document type, which will not be integrated in any report, but must be handled as separate files.
Note: In the export administration you can indicate whether the attached files should be included in the data export or not.
If required by the respective national/regional programme, briefly summarise the relevant aspects of the study including the conclusions reached. If a specific format is prescribed, upload the respective freetext template if available from the drop-down list or copy it from the corresponding document.
Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
A Cross-reference to other study or other studies can be included which are considered relevant in the interpretation of the test results, e.g. for supporting the conclusion that an effect observed was not substance-related. Indicate the respective chapter(s) and record ID(s) and enter relevant explanatory text.
Such cross-references may be useful if it is considered relevant to discuss other results at the summary level of a single study. It should be noted that the overall appraisal of results from different studies is normally done in the hazard or risk assessment.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
In the following, the online help texts for all data entry fields provided with any Endpoint study record for this IUCLID section are listed. For sections 4 to 10, these guidance notes are completely based on the so-called OECD Harmonised Templates (see Rationale behind IUCLID Endpoint Study Records - OECD harmonised templates in chapter chapter D.4.7.1 What is an Endpoint study record?)
IUCLID per se does not prescribe how detailed the study summaries should be recorded. Refer to the relevant guidance for the respective chemical regulatory programme thereof.
For technical guidance on how to manage Endpoint study records, see chapter D.4.7 How to manage Endpoint study records in sections 4 - 13. For details on data types, see chapter D.4.5 What data types are available for input fields and how are they used?
Under this main heading, fields are subsumed for identifying the purpose of the record (e.g., "key study"), the type of result (e.g., "experimental study"), data waiving indication (if any), reliability indication, and flags for indicating the regulatory purpose envisaged and/or any confidentiality restrictions. This kind of data characterise the relevance of a study summary and are therefore displayed on top of each Endpoint Study Record. For detailed guidance, refer to chapter D.4.7.7.1 Administrative data.
Indicate the bibliographic reference of the study report or publication the study summary is based on. Always enter the primary reference in the first block of fields (i.e. Sort no. = 1), if there are more than one reference to be cited. Copy this block of fields for specifying any other references related to this record (e.g. report of a preliminary study or other documentation). If results of a study report have been published, indicate the full citation of that publication(s) in addition to the reference of the original study.
Table E.200. Field Descriptions
| Reference type | Indicate the type of reference, e.g. "Study report" or "Publication". Select "Other company data" to characterise any unpublished information from a company other than a study report. Select "Grey literature" for any other unpublished information or "other:" and specify. |
| Author(s) (or transferred reference) (Author) | For ease of sorting and searchability use following convention: Surname, Initial (Example 1: White D, Ruehl KJ, Borman SA & Little J. Example 2: Hartley M & Murray W (avoid unnecessary full-stops, commas)). If no individuals are cited as authors, enter name of company or organisation or "Anon." as appropriate. Note that the complete bibliographic reference may appear in this field after migration of unstructured data from existing databases. |
| Year | Enter year of study report or publication. For a study report this field should be completed to include it in any searches, regardless of whether the complete date is given in field "Report date". |
| Title | Include the title of the report. For publications, include the title of the article of a journal or article/chapter of a book (e.g. handbook). |
| Bibliographic source | Not relevant for any study report. For publications or any other literature source (grey literature) specify the following type of information: (i) Title of scientific journal or book (e.g. if handbook); (ii) Volume of journal; (iii) Editor, publisher, place of publication for books or articles in books; (iv) Pagination. Example 1 (journal): J. Agric. Food Chem. 38: 215-227 Example 2 (handbook): In: Lyman WJ (ed.) Handbook of chemical property estimation methods. Environmental behavior of organic compounds. McGraw-Hill Book Company 15.1-15.34, New York. |
| Testing laboratory | Either manually enter the name of the testing laboratory or select it from the picklist. In either case, editing is possible. |
| Report no. | Specify the report number allocated by the testing laboratory. Note that any company-specific study number should be included in the respective field. |
| Owner company | Either manually enter the identity of the company who owns the data or select it from the picklist. In either case, editing is possible. |
| Company study no. | Specify any company study no. if there is such a number and if it is different from the report no. of the testing laboratory. Otherwise leave field empty. |
| Report date | Specify the complete date of the study report, e.g. "2005-05-12" for 12 May 2005. Note that subfield "Year" should be completed in any case for sorting and searching purposes. |
Select appropriate indication for data access. Enter "Not applicable" if the summary consists of information that is commonly accessible such as guidance on safe use.
Indicate as appropriate. Note: "yes" should be selected only if "Data submitter is data owner" or "Data submitter has Letter of Access". Options "yes, but willing to share" or "yes, but not willing to share" may be relevant for specific regulatory programmes where the submitter is requested to indicate whether he is willing to share studies (e.g. with vertebrates).
In the supplementary remarks field, include an explanation as appropriate, i.e. justification for denial of sharing the corresponding study or refer to a document attached that provides justification (e.g. "for justification see attached document X")
A cross-reference can be included to indicate that the same study is recorded in another record. Indicate the respective chapter and record ID and enter relevant explanatory text. This may be useful if specific endpoints of a given study are described in another chapter (e.g. results on reproduction toxicity in case of a combined repeated dose / reproduction toxicity study) or if more than one experiment is described by the same study report, but included in separate records.
Check with the relevant guidance document whether all the methodology details must be repeated or whether a cross-reference to the same study in another chapter may suffice.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
Include a short description of the type of information or study. Enter any details in fields "Any other information on materials and methods incl. tables" or "Any other information on results incl. tables" as appropriate..
Fill in fields for Administrative data and Data source as appropriate. If other data from the same study are provided in another chapter, include a reference in field "Same study described in chapter".
Indicate according to which test guideline the study was conducted. If no test guideline was explicitly followed, but the methodology used is equivalent or similar to a specific guideline, you can indicate so in the "Qualifier" subfield preceding the field "Guideline".
Copy this block of fields for specifying more than one guideline (e.g. US EPA in addition to OECD guideline).
Table E.201. Field Descriptions
| Qualifier | Select appropriate qualifier, i.e. - "according to" (if a given test guideline was followed); - "equivalent or similar to" (if no test guideline was explicitly followed, but the methodology is equivalent or similar to a specific guideline); - "no guideline followed" (if none of above qualifiers apply. If so, fill in field "Principles of method if other than guideline"); - "no guideline available" (if so, fill in field "Principles of method if other than guideline"). - "no guideline required" (if so, fill in field "Principles of method if other than guideline"). |
| Guideline | Select the applicable test guideline, e.g. "OECD Guideline xxx". If the test guideline used is not listed, choose "other guideline:" and specify the test guideline in the related text field. In this text field, you can also enter any remarks as applicable, particularly: - To include any other title of the test guideline draft used, a subtitle, another version or update number and the year of update (For instance, different titles and/or numbers may exist for a given EU test guideline.); - To indicate if a the study was performed prior to the adoption of the test guideline specified; - To indicate if the methodology used was based on an extension of the test guideline specified. |
| Deviations from guideline (Deviations) | For robust study summaries or as requested by the regulatory programme, indicate if there are any deviations from the test guideline specified. If "yes" is selected, only briefly state relevant deviations in the supplementary remarks field (e.g. "other species used"); details should be described in the respective fields of the section MATERIALS AND METHODS. |
If no guideline was followed, include a description of the principles of the test protocol or estimated method used in the study. Details should be entered in appropriate distinct fields of section MATERIALS AND METHODS if available. Also provide a justification for using this method if appropriate.
If an estimation method was used (to be indicated in field "Test result type") state the equation(s) and/or computer software or other methods applied to calculate the value(s).
Indicate whether the study was conducted following Good Laboratory Practice or not. Select "yes (incl. certificate)" if a GLP certificate of a test facility is available. Select "yes" if a GLP compliance statement is available, but no information on a GLP certificate. You can give an explanation in the supplementary remarks field, e.g. for explaining why GLP was not complied with or for specifying which (national) GLP was followed.
Indicate if the test material used in the study is equivalent to the submission substance identity. If "yes" is selected, the corresponding identity is automatically entered in the subsequent block of fields "Test material identity".
If "no" is selected, identify the test material in the subsequent block of fields "Test material identity". In this case, also make sure that the information entered in field "Study result type" is consistent, i.e. "read-across from supporting substance (structural analogue or surrogate)".
NOTE: If a completed record is used for another submission, you may have to update both fields "Study result type" and "Test material equivalent to submission substance identity".
If the identity of the test material used for this study is not included in this block of fields automatically, indicate the identity for one or more appropriate identifiers, e.g. CAS number, CAS name, IUPAC name. Copy this block of fields as appropriate.
If another than the submission substance identity was selected erraneously, go back to field "Test material equivalent to submission substance identity" and select "yes". This will prompt automatic entry of the respective identifiers.
Table E.202. Field Descriptions
| Identifier | Select an appropriate identifier from drop-down list, e.g. "CAS number". Use "Other:" and specify, if identity according to a standard identifier is not known or if an additional chemical name or number is provided. |
| Identity | Select the corresponding substance identity from drop-down list or enter manually if the identity is not available from the list or if no list is provided for the type of identifier selected. |
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Note that any information that can be claimed confidential should be included in the subsequent field "Confidential details on test material".
Explanations:
- Name of test material (as cited in study report): only if different from any other identifiers provided in the preceding fields.
- Molecular formula (if other than submission substance): specify
- Molecular weight (if other than submission substance): specify
- Smiles notation (if other than submission substance): provide if available
- InChl (if other than submission substance): provide if available
- Structural formula attached as image file (if other than submission substance): see Fig.: only if different from submission substance. Indicate Fig. no. if a file is attached in field "Attached document", e.g. state "see Fig. 1".
- Substance type: indicate whether pure active substance, technical product, formulation or other.
- Physical state: indicate "gas", "solid" or "liquid" only if different from submission substance or if substance can occur in different physical states.
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Isomers composition: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: provide if available
- Expiration date of the lot/batch: provide if available
- Radiochemical purity (if radiolabelling): specify if applicable
- Specific activity (if radiolabelling): specify if applicable
- Locations of the label (if radiolabelling): specify if applicable
- Expiration date of radiochemical substance (if radiolabelling): specify if applicable
- Storage condition of test substance: specify if applicable
- Stability under test conditions: indicate if available
Enter any confidential information on the test material in this separate field. Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Explanations:
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: : provide if available
- Expiration date of the lot/batch: : provide if available
- Isomers composition: specify if applicable
In this field, you can enter any information on materials and methods, for which no distinct field is available, or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
In this field, you can enter any other remarks on results. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: Both the "Materials and methods" section and "Results" section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
In this field, you can enter any overall remarks or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Attach any background document that cannot be inserted in any rich text editor field, particularly image files (e.g. an image of a structural formula).
Copy this block of fields for attaching more than one file.
Table E.203. Field Descriptions
| Attached document | Upload file by clicking the upload icon. As appropriate, enter any additional information, e.g. language. The file name is displayed after uploading the document. |
| Remarks | As appropriate, include remarks, e.g. a short description of the content of the attached document if the file name is not self-explanatory. |
If required, an electronic copy of the full study report can be attached as WORD, pdf or other document type, which will not be integrated in any report, but must be handled as separate files.
Note: In the export administration you can indicate whether the attached files should be included in the data export or not.
If required by the respective national/regional programme, briefly summarise the relevant aspects of the study including the conclusions reached. If a specific format is prescribed, upload the respective freetext template if available from the drop-down list or copy it from the corresponding document.
Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
A Cross-reference to other study or other studys can be included which are considered relevant in the interpretation of the test results, e.g. for supporting the conclusion that an effect observed was not substance-related. Indicate the respective chapter(s) and record ID(s) and enter relevant explanatory text.
Such cross-references may be useful if it is considered relevant to discuss other results at the summary level of a single study. It should be noted that the overall appraisal of results from different studys is normally done in the hazard or risk assessment.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.